BIOTECHNOLOGY J50 rar-B-
RAW SEQUENCE LISTING
ERROR REPORT
The Biotechnology Systems Branch of the Scientific and Technical Information
Center (STIC) detected errors when processing the following computer readable
form:
Application Serial Number: 0
Source:
Date Processed by STIC: j 2>
THE ATTACHED PRINTOUT EXPLAINS DETECTED ERRORS.
PLEASE FORWARD THIS INFORMATION TO THE APPLICANT BY EITHER:
1) INCLUDING A COPY OF THIS PRINTOUT IN YOUR NEXT COMMUNICATION TO THE
APPLICANT, WITH A NOTICE TO COMPLY or,
2) TELEPHONING APPLICANT AND FAXING A COPY OF THIS PRINTOUT, WITH A.
NOTICE TO COMPLY
FOR CRF SUBMISSION QUESTIONS, PLEASE CONTACT MARK SPENCER, 703-308-4212.
FOR SEQUENCE RULES INTERPRETATION, PLEASE CONTACT ROBERT WAX, 703-308-4216.
PATENTTN 2.1 e-mail help: patin21help(q),uspto.gov or phone 703-306-4119 (R. Wax)
PATENTED 3.0 e-mail help: patin3help@uspto.gov or phone 703-306-4119 (R. Wax)
TO REDUCE ERRORED SEQUENCE LISTINGS, PLEASE USE THE CHECKER
VERSION 3.0 PROGRAM. ACCESSIBLE THROUGH THE U.S. PATENT AND
TRADEMARK OFFICE WEBSITE. SEE BELOW:
Checker Version 3.0
The Checker Version 3.0 application is a state-of the-art Windows based software program
employing a logical and intuitive user-interface to check whether a sequence listing is in
compliance with format and content rules. Checker Version 3.0 works for sequence listings
generated for the original version of 37 CFR §§1.821 - 1.825 effective October 1, 1990 (old
rules) and the revised version (new rules) effective July 1, 1998 as well as World Intellectual
Property Organization (WIPO) Standard ST.25.
Checker Version 3 .0 replaces the previous DOS-based version of Checker, and is Y2K-
compliant. Checker allows public users to check sequence listings in Computer Readable form
(CRF) before submitting them to the United States Patent and Trademark Office (USPTO).
Use of Checker prior to filing the sequence listing is expected to result in fewer errored sequence
listings, thus saving time and money.
Checker Version 3.0 can be down loaded from the USPTO website at the following address:
httpV/vvww.uspto.govAveb/offices/pac/checker
Raw Sequence Listing Error Summary
ERROR DETECTED SUCCESTEP CORRECTION SERIAL NUMBER:
ATTN: NEW RULES CASES: PLEASE DISRECARD ENGLISH "ALPHA" HEADERS, WHICH WERE INSERTED BY PTO SOFT
1 Wrapped Nuclei es
Wrapped Amino;
The number/text at the end or etch line "wrapped" down to the next line. This may occur if your file
was retrieved in * word processor after erciling it. Please adjust your right margin to .3; this will
prevent "wrapping."
_lnvalid Line Length The rules require that a line not exceed 72 characters in length. This includes white spaces.
3 Misaligned Amino
Numbering
4 Non-ASCII
^Variable Length
^Patentln 2.0
"bug"
The numbering under each J* amino acid is misaligned. Do not use tab codes between numbers;
use space characters, instead.
The submitted file was not saved in ASCII(DOS) text, as required by the Sequence- Rules. Please
ensure your subsequent submission b laved in ASCII teit.
., y
Sequences) contain n's or Xaa'i representing more than, one rcstoue. Per Sequence Rules,
each n orXaa can only represent a single residue. Please present the maximum number of each
residue having variable length and indicate in the <220>-<223> section that some may be missing.
A "bug" in Patentln version 2.0 has caused Aie <220>-<223>«ection to be missing from amino acid
sequencers) . NormaHy, Palcrttjh would 1 automatically generate this section from the
previously coded nucleic acid sequenced Please manually copy th« relevant <220>-<223> section to
the subsequent amino acid sequence. This applies to the mandatory <220>-<223> sections for
Artificial »r I/iiiCiivtVii sequence;.
_Sfcipped Sequences Sequence(s)
(OLD RULES)
missing. Crintentional, please insert the following lines for each skipped sequence:
(2) INFORMATION FOR SEQ ID NO:X: (insert SEQ ID NO where "X" is shown)
(i) SEQUENCE CHARACTERISTICS: (Do not insert any subheadings under this heading)
(xi) SEQUENCE DESCRIPTION:SEQ ID NO;X: (insert SEQID NO where "X" is shown)
This sequence is intentionally skipped
Please also adjust the "(ii) NUMBER OF SEQUENCES:" response to Include the skipped sequences.
8 Skipped Sequences'*^.
(NEW RULES)
Sequences)
missing. If Intentional, please insert the following lines for each skipped sequence.
_Use of n's or Xaa's
(NEW RULES)
0^) U
IO_t_I_Jnvalid<213>
Response
<2 10> sequence id number
<400> sequence id number
000
Use of n's and/or Xaa's have been detected in the Sequence Listing.
Per 1.823 of Sequence Rules, use of<220>-<223> is MANDATORY if n's or Xaa's are present.
In <220> to <223> section, please explain location of n or X»a, and which residue n or Xai represents.
Per 1 .823 of Sequence Rules, the only valid <2 1 3> responses are: Unknown, Artificial Sequence, or
scientific name (Genus/species). <220>-<223> section is required when <213> response is Unknown or
is Artificial Sequence fa fys t OsWi^j/yxC j
II
Use of<220>
Sequences) _
missing the <220> "Feature" and associated numeric identifiers and responses.
12
_PatentIn 2.0
"bug"
Use of <220> to<223> is MANDATORY if<213> "Organism" response is "Artificial Sequence" or
"Unknown." Please explain source of genetic material in <220> to <223> section.
(See "Federal Register." 06/01/1998, Vol. «3, No. 104, pp. 2963 f-32) (Sec. 1.823 ofSequence Rules)
Please do not use "Copy to Disk" function of Patentln version 2.0. This causes a corrupted file,
resulting in missing mandatory numeric identifiers and responses (as indicated on raw sequence
listing). Instead, please use "File Manager" or any other manual means to copy file to floppy disk.
1 3 Misuse of-n ! n can only be used to represent a single nucleotide in a nucleic acid sequence. N is not used to represent
any value not specifically a nucleotide.
AMC/MH - Biotechnology Systems Branch -08/2 1/200 1
Page 1 of 3
OIPE
3
4
5
6
7
9
Used
10
12
fcp«> 15
17
18
20
21
23
25
27
28
29
C--> 30
32
33
34
35
36
38
39
42
43
44
45
47
48
49
50
51
53
54
C-->
RAW SEQUENCE LISTING DATE: 12/06/2001
PATENT APPLICATION: US/09/998,130 TIME: 15:31:58
Input Set : A:\es.TXT
Output Set: N:\CRF3\12062001\I998130.raw
<110> APPLICANT: Kishimoto, Takahide
Sogabe, Atsushi
Hattori, Shizuo
Oka, Masanori
Kawamura, Yoshihisa
<120> TITLE OP INVENTION: Determination Method of Biological Component and Reagent Kit
Does Not Comply
Corrected Diskette Needed
Therefor
FILE REFERENCE: 2356/10
CURRENT APPLICATION NUMBER: US/09/998,130
CURRENT FILING BATE: 2001-12-03
PRIOR APPLICATION NUMBER : JP 2000-370445
PRIOR FILING DATE: 2000-12-05
PRIOR APPLICATION NUMBER: JP 2001-96724
PRIOR FILING DATE: 2001-03-29
NUMBER OF SEQ ID NOS : 2
SOFTWARE: Patentln version 3.0
<130>
<140>
<14i>
<150>
<151>
<150>
<151>
<160>
<170>
<210> SEQ ID NO:
<211> LENGTH: 40
<212> TYPE: DN?
<213> ORGANISM^ Artificial/Unknown
<220> FEATURE:
<221> NAME/KEY: misc_f eature
<222> LOCATION: ()..()
<223> OTHER INFORMATION: Oligonucleotide designed to act as PCR primer for amplifying
human betaine-homocysteine methyltransf erase cDNA.
<400> SEQUENCE: 1
gcaattccat atgccacccg ttgggggcaa aaaggccaag 40
<210> SEQ ID NO: 2
<211> LENGTH: 40
<212> TYPE: DN
<213> ORG AN IS
<220> FEATURE
<221> NAME/KEY: misc_teature
<222> LOCATION: ()..()
<223> OTHER INFORMATION: Oligonucleotide designed to act as PCR primer for amplifying
human betaine-homocysteine methyltransf erase cDNA.
<400> SEQUENCE : 2
atcgcggatc caggctactg tgatttgaat ttttgttttt 40
file://C:\Crf3\Outhold\VsrI998 1 30.htm
12/6/01
Page 2 of 3
VERIFICATION SUMMARY DATE : 12/06/2001
PATENT APPLICATION: US/09/998,130 TIME : 15:31:59
Input Set : A:\es.TXT
Output Set: N:\CRF3\12062001\I998130.raw
L:14 M:270 C: Current Application Number differs, Replaced Current Application Number
L:15 M:271 C: Current Filing Date differs, Replaced Current Filing Date
L:30 M:220 C: Keyword misspelled or invalid format, <213> ORGANISM for SEQ ID#:1
L:45 M:220 C: Keyword misspelled or invalid format, <213> ORGANISM for SEQ ID# : 2
file://C:\Crf3\Outhold\VsrI998 1 30.htm
12/6/01